Overview

  • Product name
    Anti-REV3L antibody
  • Description
    Rabbit polyclonal to REV3L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal region of Human REV3L.

  • Positive control
    • Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    This Product recognizes the catalytic subunit of DNA polymerase zeta a nonessential enzyme that is thought to carry out translation replication and is responsible for virtually all DNA damage-induced mutagenesis and the majority of spontaneous mutagenesis.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • Catalytic subunit of DNA polymerase zeta antibody
      • Polymerase DNA zeta antibody
      • POLZ antibody
      • RAD54 like antibody
      • REV3 yeast homolog like antibody
      • Sez4 antibody
      • Yeast homolog of Rev 3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV3L antibody (ab111729)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV3L antibody (ab111729)
      ab111729 at 1/50 dilution staining REV3L in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

    References

    ab111729 has not yet been referenced specifically in any publications.

    Publishing research using ab111729? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample
    Human Cell (HEK293 cell line)
    Specification
    HEK293 cell line
    Permeabilization
    Yes - 0.5% Triton X-100
    Fixative
    Paraformaldehyde
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    Verified customer

    Submitted May 21 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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