Key features and details
- Rabbit polyclonal to Rex1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Rex1 antibody
See all Rex1 primary antibodies
DescriptionRabbit polyclonal to Rex1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
- Lysate of 293T cells overexpressing Rex1, Mouse Embryonic Stem Cells expressing biotinylated Rex1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionInvolved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation.
Tissue specificityExpressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
- Information by UniProt
- hREX-1 antibody
- Reduced Expression 1 Protein antibody
- Reduced expression protein 1 antibody
ICC/IF image of ab28141 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28141, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-Rex1 antibody (ab28141) at 0.5 µg/ml
Lane 1 : Lysate of 293T cells overexpressing Rex1
Lane 2 : Lysate of Mouse Embryonic Stem Cells expressing biotinylated Rex1
Lane 3 : Negative control for tagged protein - Lysate of Mouse Embryonic Stem Cells (endogenous protein present)
Lanes 4-7 : Lysate of various passages of Mouse Embryonic Stem Cells expressing biotinylated Rex1
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 34 kDa (possible non-specific binding), 36 kDa (possible non-specific binding), 38 kDa (possible tagged protein)
In this western blot Anti-Rex1 antibody ab28141 recognizes a band at 32kDa corresponding to the endogenous Rex1 protein and a band at 38 kDa corresponding to biotinylated Rex1. The identities of the bands at 34 and 36 kDa are unknown. It is possible that they represent non-specific binding, but it may be that they represent modified forms of the non-biotinylated endogenous Rex1. The biotinylated Rex1 protein contains an N-terminal FLAG and a 25 AA peptide substrate for biotinylation. Therefore, the biotinylated protein is expected to migrate at a slightly higher molecular weight than endogenous Rex1. The large bands at the top of the image are likely to represent non-specific binding.
Anti-Rex1 antibody (ab28141) at 1 µg/ml + 293T Cells Overexpressing Mouse Rex1 at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
The REX1 protein in these 293T cells contains an N-terminal FLAG tag. We expect the tagged protein to migrate at a higher molecular weight than endogenous REX1.
ab28141 has been referenced in 11 publications.
- Liu J et al. Deriving rabbit embryonic stem cells by small molecule inhibitors. Am J Transl Res 11:5122-5133 (2019). PubMed: 31497228
- Wang F et al. Rlim-Dependent and -Independent Pathways for X Chromosome Inactivation in Female ESCs. Cell Rep 21:3691-3699 (2017). WB, ICC/IF ; Mouse . PubMed: 29281819
- Loos F et al. Xist and Tsix Transcription Dynamics Is Regulated by the X-to-Autosome Ratio and Semistable Transcriptional States. Mol Cell Biol 36:2656-2667 (2016). PubMed: 27528619
- Chelmicki T et al. MOF-associated complexes ensure stem cell identity and Xist repression. Elife 3:e02024 (2014). PubMed: 24842875
- Peitz M et al. Cell-permeant recombinant Nanog protein promotes pluripotency by inhibiting endodermal specification. Stem Cell Res 12:680-9 (2014). PubMed: 24681518