• Product name

  • Description

    Rabbit polyclonal to Rex1
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Rex1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab28619.)

  • Positive control

    • Lysate of 293T cells overexpressing Rex1, Mouse Embryonic Stem Cells expressing biotinylated Rex1.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab28141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • Function

    Involved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation.
  • Tissue specificity

    Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • hREX-1 antibody
    • Reduced Expression 1 Protein antibody
    • Reduced expression protein 1 antibody
    • Rex-1 antibody
    • REX1 antibody
    • REX1 transcription factor antibody
    • Zfp-42 antibody
    • Zfp42 antibody
    • ZFP42 zinc finger protein antibody
    • ZFP42_HUMAN antibody
    • Zinc finger protein 42 homolog antibody
    • Zinc finger protein 754 antibody
    • ZNF754 antibody
    see all


  • ICC/IF image of ab28141 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28141, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-Rex1 antibody (ab28141) at 0.5 µg/ml

    Lane 1 : Lysate of 293T cells overexpressing Rex1
    Lane 2 : Lysate of Mouse Embryonic Stem Cells expressing biotinylated Rex1
    Lane 3 : Negative control for tagged protein - Lysate of Mouse Embryonic Stem Cells (endogenous protein present)
    Lanes 4-7 : Lysate of various passages of Mouse Embryonic Stem Cells expressing biotinylated Rex1

    Predicted band size: 32 kDa
    Observed band size: 32 kDa
    Additional bands at: 34 kDa (possible non-specific binding), 36 kDa (possible non-specific binding), 38 kDa (possible tagged protein)

    In this western blot Anti-Rex1 antibody ab28141 recognizes a band at 32kDa corresponding to the endogenous Rex1 protein and a band at 38 kDa corresponding to biotinylated Rex1. The identities of the bands at 34 and 36 kDa are unknown. It is possible that they represent non-specific binding, but it may be that they represent modified forms of the non-biotinylated endogenous Rex1. The biotinylated Rex1 protein contains an N-terminal FLAG and a 25 AA peptide substrate for biotinylation. Therefore, the biotinylated protein is expected to migrate at a slightly higher molecular weight than endogenous Rex1. The large bands at the top of the image are likely to represent non-specific binding.
  • Anti-Rex1 antibody (ab28141) at 1 µg/ml + 293T Cells Overexpressing Mouse Rex1 at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    The REX1 protein in these 293T cells contains an N-terminal FLAG tag. We expect the tagged protein to migrate at a higher molecular weight than endogenous REX1.


This product has been referenced in:

  • Wang F  et al. Rlim-Dependent and -Independent Pathways for X Chromosome Inactivation in Female ESCs. Cell Rep 21:3691-3699 (2017). WB, ICC/IF ; Mouse . Read more (PubMed: 29281819) »
  • Loos F  et al. Xist and Tsix Transcription Dynamics Is Regulated by the X-to-Autosome Ratio and Semistable Transcriptional States. Mol Cell Biol 36:2656-2667 (2016). Read more (PubMed: 27528619) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Testis, postnatal day 6)
Yes - 0.1% Triton X-100 in PBS
Testis, postnatal day 6
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Mr. Bryan Niedenberger

Verified customer

Submitted Sep 15 2016

Immunocytochemistry/ Immunofluorescence
Mouse Cell (Embryonic)
Yes - Triton X-100 (0.5%)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 02 2015

Western blot
Mouse Cell lysate - whole cell (Murine Embryonic Stem Cell Lysate)
Loading amount
40 µg
Murine Embryonic Stem Cell Lysate
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Utkarsh Kapoor

Verified customer

Submitted Dec 01 2011

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