Anti-REXO5 antibody (ab247021)
Key features and details
- Rabbit polyclonal to REXO5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-REXO5 antibody -
Description
Rabbit polyclonal to REXO5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human REXO5 aa 300-500.
Database link: Q96IC2 -
Positive control
- IHC-P: Human testis tissue. WB: REXO5 overexpression HEK-293T cell lysate. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Relevance
The exact functions of LOC81691 remain unknown. -
Cellular localization
Nucleolus -
Database links
- Entrez Gene: 81691 Human
- SwissProt: Q96IC2 Human
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Alternative names
- Putative RNA exonuclease NEF sp antibody
Images
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PFA fixed, Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling REXO5 using ab247021 at 4 µg/ml (green) in ICC/IF.
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Paraffin-embedded human testis tissue stained for REXO5 using ab247021 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium tissue stained for REXO5 using ab247021 at 1/200 dilution in immunohistochemical analysis. Low expression as expected.
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All lanes : Anti-REXO5 antibody (ab247021) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : REXO5 overexpression HEK-293T cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab247021 has not yet been referenced specifically in any publications.