Key features and details
- Rabbit polyclonal to RFC
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG
Product nameAnti-RFC antibody
DescriptionRabbit polyclonal to RFC
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Monkey
- WB: Rat brain and ovary tissue lysates; mouse brain, testis and ovary lysates; human placenta tissue lysate; HEK-293, PC-3 , 22RV1, K562, NIH/3T3 and COS-7 whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate dibasic dihydrate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa.|
FunctionTransporter for the intake of folate. Uptake of folate in human placental choriocarcinoma cells occurs by a novel mechanism called potocytosis which functionally couples three components, namely the folate receptor, the folate transporter, and a V-type H(+)-pump.
Tissue specificityPlacenta, liver, and to a much smaller extent, in lung.
Sequence similaritiesBelongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.
- Information by UniProt
- CHMD antibody
- FLOT 1 antibody
- FLOT1 antibody
All lanes : Anti-RFC antibody (ab272354) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysates
Lane 2 : Rat ovary tissue lysates
Lane 3 : Mouse brain tissue lysates
Lane 4 : Mouse testicular tissue lysates
Lane 5 : Mouse ovary tissue lysates
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysates
Lane 7 : Human placenta tissue lysates
Lane 8 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysates
Lane 10 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysates
Lane 11 : 22RV1 (human prostate carcinoma cell line) whole cell lysates
Lane 12 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysates
Lysates/proteins at 50 µg per lane.
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 65 kDa
5-20% SDS-PAGE gel.
Blocked membrane with 5% Non-fat Milk/ TBS for 1.5 hour at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272354 has not yet been referenced specifically in any publications.