Anti-RFC antibody (ab272354)
Key features and details
- Rabbit polyclonal to RFC
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-RFC antibody -
Description
Rabbit polyclonal to RFC -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human RFC aa 200-300.
Database link: P41440 -
Positive control
- WB: Rat brain and ovary tissue lysates; mouse brain, testis and ovary lysates; human placenta tissue lysate; HEK-293, PC-3 , 22RV1, K562, NIH/3T3 and COS-7 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate dibasic dihydrate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa.
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Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa. |
Target
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Function
Transporter for the intake of folate. Uptake of folate in human placental choriocarcinoma cells occurs by a novel mechanism called potocytosis which functionally couples three components, namely the folate receptor, the folate transporter, and a V-type H(+)-pump. -
Tissue specificity
Placenta, liver, and to a much smaller extent, in lung. -
Sequence similarities
Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family. -
Post-translational
modificationsHeavily glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6573 Human
- Entrez Gene: 20509 Mouse
- Entrez Gene: 29723 Rat
- Omim: 600424 Human
- SwissProt: P41440 Human
- SwissProt: P41438 Mouse
- SwissProt: Q62866 Rat
- Unigene: 84190 Human
see all -
Alternative names
- CHMD antibody
- FLOT 1 antibody
- FLOT1 antibody
see all
Images
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All lanes : Anti-RFC antibody (ab272354) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysates
Lane 2 : Rat ovary tissue lysates
Lane 3 : Mouse brain tissue lysates
Lane 4 : Mouse testicular tissue lysates
Lane 5 : Mouse ovary tissue lysates
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysates
Lane 7 : Human placenta tissue lysates
Lane 8 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysates
Lane 10 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysates
Lane 11 : 22RV1 (human prostate carcinoma cell line) whole cell lysates
Lane 12 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 65 kDa5-20% SDS-PAGE gel.
Blocked membrane with 5% Non-fat Milk/ TBS for 1.5 hour at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab272354 has been referenced in 1 publication.
- Tearle JLE et al. Targeted Single-cell Isolation of Spontaneously Escaping Live Melanoma Cells for Comparative Transcriptomics. Cancer Res Commun 3:1524-1537 (2023). PubMed: 37575281