Product nameAnti-RFC1 antibody
See all RFC1 primary antibodies
DescriptionGoat polyclonal to RFC1
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide (Human) - which represents a portion of human Replication Factor C 1 140 kDa subunit encoded in part by exons 25 and 26.
- HeLa cell nuclear extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab3566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 - 6 µg/ml.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 128 kDa.|
FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Tissue specificityWide tissue distribution. Undetectable in placental tissue.
Sequence similaritiesBelongs to the activator 1 large subunit family.
Contains 1 BRCT domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- A1 140 kDa subunit antibody
- A1 antibody
- A1 P145 Activator 1 large subunit antibody
ab3566, at a 1/500 dilution, staining Human RFC1 in HeLa cells. Cells were paraformaldehyde fixed (4% for 10min) and counter-stained with DAPI in order to show the nucleus. Please read abreview for more information.
This product has been referenced in:
- Abe T & Barber GN Cytosolic-DNA-mediated, STING-dependent proinflammatory gene induction necessitates canonical NF-?B activation through TBK1. J Virol 88:5328-41 (2014). WB ; Mouse . Read more (PubMed: 24600004) »
- Ragland RL et al. RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells. Genes Dev 27:2259-73 (2013). Read more (PubMed: 24142876) »