Anti-RFC1 antibody - C-terminal (ab229229)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-RFC1 antibody - C-terminal
See all RFC1 primary antibodies -
Description
Rabbit polyclonal to RFC1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human RFC1 (C terminal). The exact sequence is proprietary.
Database link: P35251 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates. IP: HeLa whole cell extract. ICC/IF: HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 128 kDa. | |
| IP | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. -
Tissue specificity
Wide tissue distribution. Undetectable in placental tissue. -
Sequence similarities
Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5981 Human
- Omim: 102579 Human
- SwissProt: P35251 Human
- Unigene: 507475 Human
-
Alternative names
- A1 140 kDa subunit antibody
- A1 antibody
- A1 P145 Activator 1 large subunit antibody
see all
Images
-
Western blot - Anti-RFC1 antibody - C-terminal (ab229229)All lanes : Anti-RFC1 antibody - C-terminal (ab229229) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 128 kDa5% SDS-PAGE gel.
-
Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody - C-terminal (ab229229)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RFC1 (green) using ab229229 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). Phalloidin, a cytoskeleton marker, was labeled at 1/200 dilution (red).
-
Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229229)RFC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 µg of ab229229. Western blot was performed from the immunoprecipitate using ab229229 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract 35 µg.
Lane 2: 4 µg preimmune Rabbit IgG instead of ab229229 in HeLa whole cell extract.
Lane 3: ab229229 IP in HeLa whole cell extract.
References
ab229229 has not yet been referenced specifically in any publications.
