The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Wide tissue distribution. Undetectable in placental tissue.
Belongs to the activator 1 large subunit family. Contains 1 BRCT domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Replication factor C (activator 1) 1, 145kDa antibody
Replication factor C 140 kDa subunit antibody
Replication factor C antibody
Replication factor C large subunit antibody
Replication factor C subunit 1 antibody
Replication factor C1 antibody
RF-C 140 kDa subunit antibody
RFC140 Replication Factor C 140 kDa subunit antibody
Western blot - Anti-RFC1 antibody - C-terminal (ab229229)
All lanes : Anti-RFC1 antibody - C-terminal (ab229229) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
RFC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 µg of ab229229. Western blot was performed from the immunoprecipitate using ab229229 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract 35 µg. Lane 2: 4 µg preimmune Rabbit IgG instead of ab229229 in HeLa whole cell extract. Lane 3: ab229229 IP in HeLa whole cell extract.
has not yet been referenced specifically in any publications.
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