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    rfc1-antibody-c-terminal-ab229229.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other
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Anti-RFC1 antibody - C-terminal (ab229229)

  • Datasheet
  • SDS
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Western blot - Anti-RFC1 antibody - C-terminal (ab229229)
  • Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody - C-terminal (ab229229)
  • Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229229)

Key features and details

  • Rabbit polyclonal to RFC1 - C-terminal
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RFC1 antibody - C-terminal
    See all RFC1 primary antibodies
  • Description

    Rabbit polyclonal to RFC1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RFC1 (C terminal). The exact sequence is proprietary.
    Database link: P35251

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates. IP: HeLa whole cell extract. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • A431 whole cell lysate (ab30132)
    • A431 whole cell lysate (ab7909)

Applications

Our Abpromise guarantee covers the use of ab229229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 128 kDa.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
    Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
  • Tissue specificity

    Wide tissue distribution. Undetectable in placental tissue.
  • Sequence similarities

    Belongs to the activator 1 large subunit family.
    Contains 1 BRCT domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P35251 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5981 Human
    • Omim: 102579 Human
    • SwissProt: P35251 Human
    • Unigene: 507475 Human
    • Alternative names

      • A1 140 kDa subunit antibody
      • A1 antibody
      • A1 P145 Activator 1 large subunit antibody
      • Activator 1 140 kDa subunit antibody
      • Activator 1 large subunit antibody
      • Activator 1 subunit 1 antibody
      • DNA binding Protein PO GA antibody
      • DNA-binding protein PO-GA antibody
      • MHC binding factor beta antibody
      • MHCBFB antibody
      • PO GA antibody
      • RECC1 antibody
      • Replication factor C (activator 1) 1, 145kDa antibody
      • Replication factor C 140 kDa subunit antibody
      • Replication factor C antibody
      • Replication factor C large subunit antibody
      • Replication factor C subunit 1 antibody
      • Replication factor C1 antibody
      • RF-C 140 kDa subunit antibody
      • RFC antibody
      • RFC1 antibody
      • RFC1_HUMAN antibody
      • RFC140 antibody
      • RFC140 Replication Factor C 140 kDa subunit antibody
      see all

    Images

    • Western blot - Anti-RFC1 antibody - C-terminal (ab229229)
      Western blot - Anti-RFC1 antibody - C-terminal (ab229229)
      All lanes : Anti-RFC1 antibody - C-terminal (ab229229) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 128 kDa



      5% SDS-PAGE gel.

    • Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody - C-terminal (ab229229)
      Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody - C-terminal (ab229229)

      4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RFC1 (green) using ab229229 at 1/1000 dilution in ICC/IF.

      Nuclear counterstain: Hoechst 33342 (blue). Phalloidin, a cytoskeleton marker, was labeled at 1/200 dilution (red).

    • Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229229)
      Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229229)

      RFC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 µg of ab229229. Western blot was performed from the immunoprecipitate using ab229229 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.

      Lane 1: HeLa whole cell extract 35 µg. 
      Lane 2: 4 µg preimmune Rabbit IgG instead of ab229229 in HeLa whole cell extract. 
      Lane 3: ab229229 IP in HeLa whole cell extract. 

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab229229? Please let us know so that we can cite the reference in this datasheet.

    ab229229 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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