Anti-RFC1 antibody - C-terminal (ab229229)
Key features and details
- Rabbit polyclonal to RFC1 - C-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RFC1 antibody - C-terminal
See all RFC1 primary antibodies -
Description
Rabbit polyclonal to RFC1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human RFC1 (C terminal). The exact sequence is proprietary.
Database link: P35251 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates. IP: HeLa whole cell extract. ICC/IF: HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 128 kDa. | |
| IP | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. -
Tissue specificity
Wide tissue distribution. Undetectable in placental tissue. -
Sequence similarities
Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5981 Human
- Omim: 102579 Human
- SwissProt: P35251 Human
- Unigene: 507475 Human
-
Alternative names
- A1 140 kDa subunit antibody
- A1 antibody
- A1 P145 Activator 1 large subunit antibody
see all
Images
-
Western blot - Anti-RFC1 antibody - C-terminal (ab229229)All lanes : Anti-RFC1 antibody - C-terminal (ab229229) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 128 kDa5% SDS-PAGE gel.
-
Immunocytochemistry/ Immunofluorescence - Anti-RFC1 antibody - C-terminal (ab229229)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RFC1 (green) using ab229229 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). Phalloidin, a cytoskeleton marker, was labeled at 1/200 dilution (red).
-
Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229229)RFC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 µg of ab229229. Western blot was performed from the immunoprecipitate using ab229229 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract 35 µg.
Lane 2: 4 µg preimmune Rabbit IgG instead of ab229229 in HeLa whole cell extract.
Lane 3: ab229229 IP in HeLa whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229229 has not yet been referenced specifically in any publications.
