Overview

  • Product name

  • Description

    Goat polyclonal to RFC2
  • Host species

    Goat
  • Specificity

    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Replication Factor C 2 40 kDa subunit encoded within exon 1.

  • Positive control

    • HeLa cell nuclear extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ICC/IF 1/1000.

Target

  • Function

    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
  • Involvement in disease

    Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities

    Belongs to the activator 1 small subunits family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • A1 40 kDa subunit antibody
    • A1 antibody
    • Activator 1 40 kDa subunit antibody
    • Activator 1 antibody
    • Activator 1 subunit 2 antibody
    • MGC3665 antibody
    • OTTHUMP00000160645 antibody
    • OTTHUMP00000160646 antibody
    • Replication factor C (activator 1) 2 40kDa antibody
    • Replication factor C (activator 1) 2 antibody
    • Replication factor C 2 antibody
    • Replication factor C 40 kDa subunit antibody
    • Replication factor C subunit 2 antibody
    • RF C 40 kDa subunit antibody
    • RF-C 40 kDa subunit antibody
    • RFC 2 antibody
    • RFC 40 antibody
    • RFC 40 kDa subunit antibody
    • RFC2 antibody
    • RFC2_HUMAN antibody
    • RFC40 antibody
    see all

Images

  • Sample: 50µg Hela cell nuclear extract

    anti-RFC 2 (exon 1) (ab3615) used at 1:1000 dilution

    Sample: 50µg Hela cell nuclear extract

    anti-RFC 2 (exon 1) (ab3615) used at 1:1000 dilution

  • ab3615 (1/1000) staining RFC2 in assynchronous HeLa cells by ICC/IF, using a secondary Donkey anti-goat antibody (1/200) conjugated to Alexa Fluor® 488 (green). Cells were counterstained with DAPI (red) in order to show the nucleus.

    See Abreview

References

This product has been referenced in:

  • Kawasoe Y  et al. MutSa maintains the mismatch repair capability by inhibiting PCNA unloading. Elife 5:N/A (2016). Read more (PubMed: 27402201) »
  • Nicolas A  et al. Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments. J Virol 84:8871-87 (2010). IF . Read more (PubMed: 20573815) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 02 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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