Anti-RFC2 antibody [EPR12093(B)] (ab174271)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-RFC2 antibody [EPR12093(B)]
See all RFC2 primary antibodies -
DescriptionRabbit monoclonal [EPR12093(B)] to RFC2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human RFC2 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: P35250 -
Positive control
- HepG2, K562, MCF7, 293T, and HeLa whole cell lysate (ab150035); MCF7 and K562 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12093(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab174271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
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Involvement in diseaseNote=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
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Sequence similaritiesBelongs to the activator 1 small subunits family.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5982 Human
- Entrez Gene: 19718 Mouse
- Entrez Gene: 116468 Rat
- Omim: 600404 Human
- SwissProt: P35250 Human
- SwissProt: Q9WUK4 Mouse
- SwissProt: Q641W4 Rat
- Unigene: 647062 Human
see all -
Alternative names
- A1 40 kDa subunit antibody
- A1 antibody
- Activator 1 40 kDa subunit antibody
see all
Images
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![Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)](https://www.abcam.com/ps/products/174/ab174271/Images/ab174271-199146-ab174271.jpg)
Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)All lanes : Anti-RFC2 antibody [EPR12093(B)] (ab174271) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)](https://www.abcam.com/ps/products/174/ab174271/Images/ab174271-199145-ab174271b.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)Immunofluorescent analysis of MCF7 cells labeling RFC2 with ab174271 at 1/100 dilution.
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![Flow Cytometry - Anti-RFC2 antibody [EPR12093(B)] (ab174271)](https://www.abcam.com/ps/products/174/ab174271/Images/ab174271-199144-ab174271c.jpg)
Flow Cytometry - Anti-RFC2 antibody [EPR12093(B)] (ab174271)Flow Cytometric analysis of permeabilized HeLa cells labeling RFC2 with ab174271at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Guenat D et al. DNA damage response defect in Williams-Beuren syndrome. Int J Mol Med 39:622-628 (2017). WB . Read more (PubMed: 28098859) »
