RecombinantRabMAb

Recombinant Anti-RFC2 antibody [EPR12093(B)] (ab174271)

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Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
  • Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
  • Flow Cytometry - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
  • Anti-RFC2 antibody [EPR12093(B)] (ab174271)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12093(B)] to RFC2
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-RFC2 antibody [EPR12093(B)]
    See all RFC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12093(B)] to RFC2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human RFC2 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: P35250

  • Positive control

    • HepG2, K562, MCF7, 293T, and HeLa whole cell lysate (ab150035); MCF7 and K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12093(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.

    • Involvement in disease

      Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

    • Sequence similarities

      Belongs to the activator 1 small subunits family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • A1 40 kDa subunit antibody
      • A1 antibody
      • Activator 1 40 kDa subunit antibody
      • Activator 1 antibody
      • Activator 1 subunit 2 antibody
      • MGC3665 antibody
      • OTTHUMP00000160645 antibody
      • OTTHUMP00000160646 antibody
      • Replication factor C (activator 1) 2 40kDa antibody
      • Replication factor C (activator 1) 2 antibody
      • Replication factor C 2 antibody
      • Replication factor C 40 kDa subunit antibody
      • Replication factor C subunit 2 antibody
      • RF C 40 kDa subunit antibody
      • RF-C 40 kDa subunit antibody
      • RFC 2 antibody
      • RFC 40 antibody
      • RFC 40 kDa subunit antibody
      • RFC2 antibody
      • RFC2_HUMAN antibody
      • RFC40 antibody
      see all

    Images

    • Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      All lanes : Anti-RFC2 antibody [EPR12093(B)] (ab174271) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)

      Immunofluorescent analysis of MCF7 cells labeling RFC2 with ab174271 at 1/100 dilution.

    • Flow Cytometry - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      Flow Cytometry - Anti-RFC2 antibody [EPR12093(B)] (ab174271)

      Flow Cytometric analysis of permeabilized HeLa cells labeling RFC2 with ab174271at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      Anti-RFC2 antibody [EPR12093(B)] (ab174271)

    References (1)

    Publishing research using ab174271? Please let us know so that we can cite the reference in this datasheet.

    ab174271 has been referenced in 1 publication.

    • Guenat D  et al. DNA damage response defect in Williams-Beuren syndrome. Int J Mol Med 39:622-628 (2017). WB . PubMed: 28098859

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