Overview

  • Product name
    Anti-RFC2 antibody [EPR12093(B)]
    See all RFC2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12093(B)] to RFC2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RFC2 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: P35250

  • Positive control
    • HepG2, K562, MCF7, 293T, and HeLa cell lysates; MCF7 and K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12093(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function
      The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
    • Involvement in disease
      Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similarities
      Belongs to the activator 1 small subunits family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • A1 40 kDa subunit antibody
      • A1 antibody
      • Activator 1 40 kDa subunit antibody
      • Activator 1 antibody
      • Activator 1 subunit 2 antibody
      • MGC3665 antibody
      • OTTHUMP00000160645 antibody
      • OTTHUMP00000160646 antibody
      • Replication factor C (activator 1) 2 40kDa antibody
      • Replication factor C (activator 1) 2 antibody
      • Replication factor C 2 antibody
      • Replication factor C 40 kDa subunit antibody
      • Replication factor C subunit 2 antibody
      • RF C 40 kDa subunit antibody
      • RF-C 40 kDa subunit antibody
      • RFC 2 antibody
      • RFC 40 antibody
      • RFC 40 kDa subunit antibody
      • RFC2 antibody
      • RFC2_HUMAN antibody
      • RFC40 antibody
      see all

    Images

    • Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      All lanes : Anti-RFC2 antibody [EPR12093(B)] (ab174271) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)

      Immunofluorescent analysis of MCF7 cells labeling RFC2 with ab174271 at 1/100 dilution.

    • Flow Cytometry - Anti-RFC2 antibody [EPR12093(B)] (ab174271)
      Flow Cytometry - Anti-RFC2 antibody [EPR12093(B)] (ab174271)

      Flow Cytometric analysis of permeabilized HeLa cells labeling RFC2 with ab174271at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab174271? Please let us know so that we can cite the reference in this datasheet.

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