Product nameAnti-RFC2 antibody [EPR12093(B)]
See all RFC2 primary antibodies
DescriptionRabbit monoclonal [EPR12093(B)] to RFC2
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RFC2 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: P35250
- HepG2, K562, MCF7, 293T, and HeLa whole cell lysate (ab150035); MCF7 and K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
Involvement in diseaseNote=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the activator 1 small subunits family.
- Information by UniProt
- A1 40 kDa subunit antibody
- A1 antibody
- Activator 1 40 kDa subunit antibody
All lanes : Anti-RFC2 antibody [EPR12093(B)] (ab174271) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Immunofluorescent analysis of MCF7 cells labeling RFC2 with ab174271 at 1/100 dilution.
Flow Cytometric analysis of permeabilized HeLa cells labeling RFC2 with ab174271at 1/10 dilution (red) or a rabbit IgG (negative) (green).