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Rabbit polyclonal to RFC3
Recombinant fragment, corresponding to a region within amino acids 51-353 of Human RFC3 (Uniprot ID: P40938).
HeLa whole cell lysate; HeLa cells; Human gastric carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
1/100 - 1/1000.
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
Belongs to the activator 1 small subunits family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
A1 38 kDa subunit antibody
Activator 1 38 kDa subunit antibody
Activator 1 subunit 3 antibody
Western blot - Anti-RFC3 antibody (ab154899)
Anti-RFC3 antibody (ab154899) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 41 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody (ab154899)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling RFC3 with ab154899 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC3 antibody (ab154899)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue labeling RFC3 with ab154899 at 1/500 dilution.
This product has been referenced in:
et al. Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT. Transl Oncol 10:1-9 (2017).
Read more (PubMed: 27888707) »
et al. Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants. Proc Natl Acad Sci U S A 114:3720-3725 (2017).
Read more (PubMed: 28320935) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"