Anti-RFC3 antibody (ab3619)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-RFC3 antibody
See all RFC3 primary antibodies -
Description
Goat polyclonal to RFC3 -
Host species
Goat -
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human RFC3.
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Positive control
- HeLa cell nuclear extract.
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General notes
Previously labelled as RFC5.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab3619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent dilution. | |
| WB | 1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. -
Sequence similarities
Belongs to the activator 1 small subunits family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5985 Human
- Omim: 600407 Human
- SwissProt: P40937 Human
- Unigene: 731908 Human
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Alternative names
- 36 kDa subunit antibody
- A1 36 antibody
- A1 36 kDa subunit antibody
see all
Images
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Western blot - Anti-RFC3 antibody (ab3619)Sample: 50µg Hela cell nuclear extract
Anti-RFC3 (ab3619) used at 1:1000 dilution
Sample: 50µg Hela cell nuclear extract
Anti-RFC3 (ab3619) used at 1:1000 dilution
References
This product has been referenced in:
- Overmeer RM et al. Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment. Mol Cell Biol 30:4828-39 (2010). ICC/IF . Read more (PubMed: 20713449) »
- Schad A et al. Expression of catalase mRNA and protein in adult rat brain: detection by nonradioactive in situ hybridization with signal amplification by catalyzed reporter deposition (ISH-CARD) and immunohistochemistry (IHC)/immunofluorescence (IF). J Histochem Cytochem 51:751-60 (2003). Read more (PubMed: 12754286) »
