Anti-RFC3 antibody (ab3619)

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Western blot - Anti-RFC3 antibody (ab3619)

    Key features and details

    • Goat polyclonal to RFC3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-RFC3 antibody
      See all RFC3 primary antibodies

    • Description

      Goat polyclonal to RFC3

    • Host species

      Goat

    • Specificity

      We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Human RFC3.

    • Positive control

      • HeLa cell nuclear extract.

    • General notes

      Previously labelled as RFC5. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • Storage buffer

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3619 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Chimpanzee
    Gorilla
    Orangutan
    Application Abreviews Notes
    WB
    1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
    Notes
    WB
    1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).

    Target

    • Function

      The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.

    • Sequence similarities

      Belongs to the activator 1 small subunits family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • 36 kDa subunit antibody
        • A1 36 antibody
        • A1 36 kDa subunit antibody
        • Activator 1 36 kDa subunit antibody
        • Activator 1 antibody
        • Activator 1 subunit 5 antibody
        • MGC1155 antibody
        • Replication factor C (activator 1) 5 (36.5kD) antibody
        • Replication factor C 36 kDa subunit antibody
        • Replication factor C 5 isoform 1 antibody
        • Replication Factor C antibody
        • Replication factor C subunit 5 antibody
        • replication factor C, 36-kDa subunit antibody
        • RF-C 36 kDa subunit antibody
        • RFC 36.5 kD subunit antibody
        • RFC 5 antibody
        • RFC3, yeast, homolog of antibody
        • RFC36 antibody
        • rfc5 antibody
        • RFC5_HUMAN antibody
        see all

      Images

      • Western blot - Anti-RFC3 antibody (ab3619)
        Western blot - Anti-RFC3 antibody (ab3619)

        Sample: 50µg Hela cell nuclear extract

        Anti-RFC3 (ab3619) used at 1:1000 dilution

        Sample: 50µg Hela cell nuclear extract

        Anti-RFC3 (ab3619) used at 1:1000 dilution

      References (2)

      Publishing research using ab3619? Please let us know so that we can cite the reference in this datasheet.

      ab3619 has been referenced in 2 publications.

      • Overmeer RM  et al. Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment. Mol Cell Biol 30:4828-39 (2010). ICC/IF . PubMed: 20713449
      • Schad A  et al. Expression of catalase mRNA and protein in adult rat brain: detection by nonradioactive in situ hybridization with signal amplification by catalyzed reporter deposition (ISH-CARD) and immunohistochemistry (IHC)/immunofluorescence (IF). J Histochem Cytochem 51:751-60 (2003). PubMed: 12754286

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