Overview

  • Product name

  • Description

    Rabbit polyclonal to RFC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Pig, Chimpanzee, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide (Human) - which represents a portion of human Replication Factor C 4, 37 kDa subunit encoded within exon 2.

  • Positive control

    • Nuclear extract from HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP Use a concentration of 2 - 4 µg/ml.

Target

  • Function

    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.
  • Sequence similarities

    Belongs to the activator 1 small subunits family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • A1 37 antibody
    • A1 37 kDa subunit antibody
    • Activator 1 37 antibody
    • Activator 1 37 kDa subunit antibody
    • Activator 1 subunit 4 antibody
    • Replication factor C 37 antibody
    • Replication factor C 37 kDa subunit antibody
    • Replication factor C subunit 4 antibody
    • Replication factor C4 antibody
    • RF-C 37 kDa subunit antibody
    • RFC 37 antibody
    • RFC37 antibody
    • RFC4 replication factor C (activator 1)4 37kDa antibody
    • RfC4 antibody
    • RFC4_HUMAN antibody
    see all

Images

  • Detection of Human RFC 4 by Western Blot and Immunoprecipitation using Affinity Purified Rabbit anti-RFC 4 (ab3854).

    Sample: Nuclear Extract from HeLa cells.

    IP - 300µg; WB - 20µg

    Antibody: Affinity Purified anti-RFC 4 (ab3854)

    IP - 2µg; WB - 0.5µg/ml

    Detection: ECL, 10 minute exposure

References

ab3854 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab3854.
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