Overview

  • Product name

  • Description

    Rabbit polyclonal to RFC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 18 and 293 of Human RFC4 (NP_002907)

  • Positive control

    • 293T, A431, H1299, HeLa, HepG, Molt-4, Raji cell lysates. A549 cells. MDAMB-468 Xenograft

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.
  • Sequence similarities

    Belongs to the activator 1 small subunits family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • A1 37 antibody
    • A1 37 kDa subunit antibody
    • Activator 1 37 antibody
    • Activator 1 37 kDa subunit antibody
    • Activator 1 subunit 4 antibody
    • Replication factor C 37 antibody
    • Replication factor C 37 kDa subunit antibody
    • Replication factor C subunit 4 antibody
    • Replication factor C4 antibody
    • RF-C 37 kDa subunit antibody
    • RFC 37 antibody
    • RFC37 antibody
    • RFC4 replication factor C (activator 1)4 37kDa antibody
    • RfC4 antibody
    • RFC4_HUMAN antibody
    see all

Images

  • All lanes : Anti-RFC4 antibody (ab96852) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab96852 at 1/200 dilution. Lower image merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using ab96852 at 1/100 dilution.

References

ab96852 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab96852.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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