Western blot abreview for Anti-RFC4 antibody [EP14557] - C-terminal

Excellent
Abreviews
Application
Western blot
Sample
Xenopus laevis Cell lysate - nuclear (XTC)
Gel Running Conditions
Reduced Denaturing (5-15)
Loading amount
3000 cells
Specification
XTC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Pierce/Fisher
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
XTC cytosol
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 40 kDa
Positive control
Hela whole cell lysate

Additional data

Additional Notes
I originally thought the star rating was for xenopus only (what I was testing) for human it worked great. Sorry about that Western blot lanes: 1. HeLa whole cell lysate 2. XTC whole cell lysate 3. XTC nuclei 4. XTC cytosol Bands from bottom to top H2B, Rfc4, Tubulin, Smc3
Abcam response
Thank you for participating in our AbTrial program for untested species. We appreciate the feedback!

Dawn Bender

Verified customer

Submitted Oct 16 2017

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