Recombinant Anti-RFC4 antibody [EPR14556] (ab182145)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14556] to RFC4
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RFC4 antibody [EPR14556]
See all RFC4 primary antibodies -
Description
Rabbit monoclonal [EPR14556] to RFC4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela, HepG2, 293T or K562 lysate, HeLa or HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14556 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa.
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ICC/IF |
1/250.
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IP |
1/50.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa. |
ICC/IF
1/250. |
IP
1/50. |
Target
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Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. -
Sequence similarities
Belongs to the activator 1 small subunits family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5984 Human
- Omim: 102577 Human
- SwissProt: P35249 Human
- Unigene: 714318 Human
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Alternative names
- A1 37 antibody
- A1 37 kDa subunit antibody
- Activator 1 37 antibody
see all
Images
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All lanes : Anti-RFC4 antibody [EPR14556] (ab182145) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : K562 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution -
Detection of Human RFC4 by Western Blot of Immunoprecipitate. HeLa whole cell lysate loaded, ab182145 used for blotting immunoprecipitated RFC4 at a 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution.
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Immunofluorescent staining of HepG2 cells(right) using anti-RFC4 Alexa Fluor® 555 RabMAB and HepG2 cells (left) using Dapi (blue)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab182145 has been referenced in 3 publications.
- Kang MS et al. Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes. Nat Commun 10:2420 (2019). PubMed: 31160570
- Collin G et al. Transcriptional repression of DNA repair genes is a hallmark and a cause of cellular senescence. Cell Death Dis 9:259 (2018). PubMed: 29449545
- Xiang J et al. Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer. J Transl Med 12:320 (2014). PubMed: 25407051