Product nameRFC4 overexpression 293T lysate (whole cell)
ab94287 is a 293T cell transfected lysate in which Human RFC4 has been transiently over-expressed using a pCMV-RFC4 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. Similarity: Belongs to the activator 1 small subunits family.
Our Abpromise guarantee covers the use of ab94287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94287 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-RFC4 antibody (ab57917) at 1/500 dilution
Lane 1 :
RFC4 overexpression 293T lysate (whole cell) (ab94287)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94287 has not yet been referenced specifically in any publications.