Overview

  • Product name

    Anti-RFESD antibody
  • Description

    Rabbit polyclonal to RFESD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-141 of Human RFESD (Q8TAC1).

  • Positive control

    • Human colon carcinoma tissue; A549, H1299, HCT116, MCF7 and Mouse brain whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or using Tris-EDTA buffer (pH8.0).

Target

Images

  • Anti-RFESD antibody (ab127901) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 18 kDa



    15% SDS Page
  • All lanes : Anti-RFESD antibody (ab127901) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 18 kDa



    15% SDS Page
  • ab127901, at 1/500 dilution, staining RFESD in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

References

ab127901 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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