Overview

  • Product name
  • Description
    Rabbit polyclonal to RFP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Immunogen

    Recombinant full length protein corresponding to RFP. Corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

  • Positive control
    • IHC: DsRed transgenic mouse retina tissue; Mouse lung and embryonic myocardial tissues; Rabbit Hair follicle. WB: RFP protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50000.
WB 1/1000 - 1/50000.
IHC-P 1/200 - 1/20000.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • All lanes : Anti-RFP antibody (ab124754) at 1 µg/ml

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 10 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
    Lane 3 : CHO-K1 (chinese hamster ovary cell line) cell lysate at 10 µg
    Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate at 10 µg
    Lane 5 : A431 (human epidermoid carcinoma cell line) cell lysate at 10 µg
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
    Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 10 µg
    Lane 8 : E-coli HCP Control at 10 µg
    Lane 9 : FLAG Positive Control Lysate at 10 µg
    Lane 10 : Red Fluorescent Protein at 0.05 µg
    Lane 11 : Green Fluorescent Protein at 0.05 µg
    Lane 12 : Glutathione-S-Transferase Protein at 0.05 µg
    Lane 13 : Maltose Binding Protein at 0.05 µg
  • All lanes : Anti-RFP antibody (ab124754) at 1/1000 dilution

    Lane 1 : RFP with Blocking buffer
    Lane 2 : Human IgG with Blocking buffer
    Lane 3 : Goat IgG with Blocking buffer
    Lane 4 : Mouse IgG with Blocking buffer

    Lysates/proteins at 0.05 µg per lane.

    Secondary
    All lanes : Rabbit peroxidase conjugate at 1/40000 dilution

    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • PFA-fixed DsRed transgenic mouse retina tissue stained for RFP using ab124754 at 1/100 dilution in immunohistochemical analysis.

    Blocking: 3% BSA, 0.3% Triton.

    Secondary antibody: Alexa488 secondary antibody at 1:10,000 for 4 hours at RT.

     

  • Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse lung sections, labelling RFP with ab124754 at 1/50 dilution for 1 hour at room temperature. The secondary used was a rabbit fluorescein conjugate at 1/250 incubated for 1 hour at room temperature.

  • Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse embryonic myocardial tissue, labelling RFP with ab124754 at a 1/50 dilution for 1 hour at room temperature. The secondary used was a a rabbit fluorescein conjugate at a 1/250 dilution for 1 hour at room temperature. Counterstain is an expression of RFP in red Tomato transgenic mice.

  • Immunohistochemical immunofluorescence analysis of 0.5% PFA fixed Rabbit Hair folicle section labelling RFP using ab124754 at a dilution of 10 ug/mL for 1 hour at room temperature. The secondary used was a blue rabbit fluorescein conjugate at a 1/10,000 for 45 mins at room temperature. A counterstain against HOP-derived cells was used (red).

References

This product has been referenced in:
See all 5 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
None
Permeabilization
No
Specification
Lung
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

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Verified customer

Submitted Mar 30 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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