Anti-RFP antibody (ab124754)
Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RFP antibody
See all RFP primary antibodies -
Description
Rabbit polyclonal to RFP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to RFP. Corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/50000.
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IHC-P | (2) |
1/200 - 1/20000.
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Notes |
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WB
1/1000 - 1/50000. |
IHC-P
1/200 - 1/20000. |
Target
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Relevance
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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All lanes : Anti-RFP antibody (ab124754) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 10 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 3 : CHO-K1 (chinese hamster ovary cell line) cell lysate at 10 µg
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate at 10 µg
Lane 5 : A431 (human epidermoid carcinoma cell line) cell lysate at 10 µg
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 10 µg
Lane 8 : E-coli HCP Control at 10 µg
Lane 9 : FLAG Positive Control Lysate at 10 µg
Lane 10 : Red Fluorescent Protein at 0.05 µg
Lane 11 : Green Fluorescent Protein at 0.05 µg
Lane 12 : Glutathione-S-Transferase Protein at 0.05 µg
Lane 13 : Maltose Binding Protein at 0.05 µg -
All lanes : Anti-RFP antibody (ab124754) at 1/1000 dilution
Lane 1 : RFP with Blocking buffer
Lane 2 : Human IgG with Blocking buffer
Lane 3 : Goat IgG with Blocking buffer
Lane 4 : Mouse IgG with Blocking buffer
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : Rabbit peroxidase conjugate at 1/40000 dilution
Observed band size: 30 kDa why is the actual band size different from the predicted? -
PFA-fixed DsRed transgenic mouse retina tissue stained for RFP using ab124754 at 1/100 dilution in immunohistochemical analysis.
Blocking: 3% BSA, 0.3% Triton.
Secondary antibody: Alexa488 secondary antibody at 1:10,000 for 4 hours at RT.
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Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse lung sections, labelling RFP with ab124754 at 1/50 dilution for 1 hour at room temperature. The secondary used was a rabbit fluorescein conjugate at 1/250 incubated for 1 hour at room temperature.
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Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse embryonic myocardial tissue, labelling RFP with ab124754 at a 1/50 dilution for 1 hour at room temperature. The secondary used was a a rabbit fluorescein conjugate at a 1/250 dilution for 1 hour at room temperature. Counterstain is an expression of RFP in red Tomato transgenic mice.
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Immunohistochemical immunofluorescence analysis of 0.5% PFA fixed Rabbit Hair folicle section labelling RFP using ab124754 at a dilution of 10 ug/mL for 1 hour at room temperature. The secondary used was a blue rabbit fluorescein conjugate at a 1/10,000 for 45 mins at room temperature. A counterstain against HOP-derived cells was used (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab124754 has been referenced in 21 publications.
- Empl L et al. Selective plasticity of callosal neurons in the adult contralesional cortex following murine traumatic brain injury. Nat Commun 13:2659 (2022). PubMed: 35551446
- Le N et al. Ectopic insert-dependent neuronal expression of GFAP promoter-driven AAV constructs in adult mouse retina. Front Cell Dev Biol 10:914386 (2022). PubMed: 36200040
- Luecke IW et al. Viral vector gene delivery of the novel chaperone protein SRCP1 to modify insoluble protein in in vitro and in vivo models of ALS. Gene Ther N/A:N/A (2021). PubMed: 34239068
- Reuther P et al. Persistent RNA virus infection is short-lived at the single-cell level but leaves transcriptomic footprints. J Exp Med 218:N/A (2021). PubMed: 34398180
- Neuhöfer P et al. Acinar cell clonal expansion in pancreas homeostasis and carcinogenesis. Nature 597:715-719 (2021). PubMed: 34526722