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IHC-P with mouse mammary gland
tissue with RFP only
tissue with GFP only
sees weak staining in GFP-only tissue, but we had guaranteed that the antibody does not crossreact with GFP
2nd alone does not give any staining
RFP gives strong staining - this is OK
Ab: 1/100, 1/500, 1/1000 (still sees some GFP staining, but RFP staining also becomes less intense)
block: goat serum
2nd: goat anti-rabbit + DAB
for ab124754: check if cross-reactivity with GFP has been tested
Asked on Nov 19 2012
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1206764 with ab124754.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Nov 19 2012