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IHC-P with mouse mammary gland
tissue with RFP only
tissue with GFP only
sees weak staining in GFP-only tissue, but we had guaranteed that the antibody does not crossreact with GFP
2nd alone does not give any staining
RFP gives strong staining - this is OK
Ab: 1/100, 1/500, 1/1000 (still sees some GFP staining, but RFP staining also becomes less intense)
block: goat serum
2nd: goat anti-rabbit + DAB
for ab124754: check if cross-reactivity with GFP has been tested
Asked on Nov 20 2012
The lab let me know the following information regarding GFP crossreactivity of ab124754:
The antibody has been tested for cross reactivity to a His-tagged EGFP and untagged YFP and no cross reactivity was detected by either ELISA or western blot.
Answered on Nov 20 2012