Anti-RFP antibody (ab152123)
Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-RFP antibody
See all RFP primary antibodies -
Description
Rabbit polyclonal to RFP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length RFP.
-
Positive control
- RFP-transfected HeLa cells; RFP-transfected 293T lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000 - 1/20000. Predicted molecular weight: 27 kDa.
|
|
IP |
1/10000.
|
|
ICC/IF |
1/5000.
|
Notes |
---|
WB
1/5000 - 1/20000. Predicted molecular weight: 27 kDa. |
IP
1/10000. |
ICC/IF
1/5000. |
Target
-
Relevance
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
-
All lanes : Anti-RFP antibody (ab152123) at 1/10000 dilution
Lane 1 : Non-transfected 293T whole cell lysate
Lane 2 : GFP-transfected 293T whole cell lysate
Lane 3 : RFP-transfected 293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Immunoprecipitation of RFP-tagged protein.
HeLa transfected lysates were subjected to immunoprecipitation using:
Lane 1: 15 µg of HeLa transfected lysate
Lane 2: normal rabbit IgG
Lane 3: 2.5 µg ab152123
Detection ab152123 at 1/10000 dilution -
Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123: (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab152123 has been referenced in 3 publications.
- Zhou Y et al. Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1. J Virol 95:e0015321 (2021). PubMed: 34287041
- Lu X et al. Lentivirus-mediated IL-10-expressing Bone Marrow Mesenchymal Stem Cells promote corneal allograft survival via upregulating lncRNA 003946 in a rat model of corneal allograft rejection. Theranostics 10:8446-8467 (2020). PubMed: 32724480
- Joseph SR et al. Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos. Elife 6:N/A (2017). IP . PubMed: 28425915