Overview

  • Product name
  • Description
    Rabbit polyclonal to RFP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Immunogen

    Recombinant full length RFP.

  • Positive control
    • RFP-transfected HeLa cells; RFP-transfected 293T lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab152123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 27 kDa.
IP 1/10000.
ICC/IF 1/5000.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • All lanes : Anti-RFP antibody (ab152123) at 1/10000 dilution

    Lane 1 : Non-transfected 293T whole cell lysate
    Lane 2 : GFP-transfected 293T whole cell lysate
    Lane 3 : RFP-transfected 293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 27 kDa



    12% SDS PAGE
  • Immunoprecipitation of RFP-tagged protein.
    HeLa transfected lysates were subjected to immunoprecipitation using:
    Lane 1: 15 µg of HeLa transfected lysate
    Lane 2: normal rabbit IgG
    Lane 3: 2.5 µg ab152123
    Detection ab152123 at 1/10000 dilution
  • Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123: (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.

References

This product has been referenced in:
  • Joseph SR  et al. Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos. Elife 6:N/A (2017). IP . Read more (PubMed: 28425915) »

See 1 Publication for this product

Customer reviews and Q&As

The transfected protein was RFP-tagged, so the molecular weight observed in the WB on the datasheet is the molecular weight of RFP (27 kDa) + the molecular weight of the tagged protein, resulting in a total tagged protein molecular weight of ˜50 kDa. T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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