Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB, IP, ICC/IF
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab152123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 27 kDa.|
RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
All lanes : Anti-RFP antibody (ab152123) at 1/10000 dilution
Lane 1 : Non-transfected 293T whole cell lysate
Lane 2 : GFP-transfected 293T whole cell lysate
Lane 3 : RFP-transfected 293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE
Immunoprecipitation of RFP-tagged protein.
HeLa transfected lysates were subjected to immunoprecipitation using:
Lane 1: 15 µg of HeLa transfected lysate
Lane 2: normal rabbit IgG
Lane 3: 2.5 µg ab152123
Detection ab152123 at 1/10000 dilution
Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123: (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.
ab152123 has been referenced in 1 publication.
- Joseph SR et al. Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos. Elife 6:N/A (2017). IP . PubMed: 28425915