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You show a western blot with a band in the third lane with the size around 50 kDa (same size in the IP western blot image) but "Predicted band size" says "27 kDa". Is this an unspecific band then or is your RFP molecule tagged to another protein?
Asked on Dec 07 2016
The transfected protein was RFP-tagged, so the molecular weight observed in the WB on the datasheet is the molecular weight of RFP (27 kDa) + the molecular weight of the tagged protein, resulting in a total tagged protein molecular weight of ˜50 kDa. Thus, the indicated band in the western blots on the datasheet is specific.
Answered on Dec 07 2016