Anti-RFP antibody (ab28664)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameAnti-RFP antibody
See all RFP primary antibodies -
DescriptionRabbit polyclonal to RFP
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Host speciesRabbit
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Specificityab28664 detects RFP tagged proteins. This antibody is specific for the RFP tag. It shows very low reactivity to GFP or GFP tagged proteins. ab28664 non-specifically detects a 45 kDa protein from E. coli extracts. This non-specific cross-reactivity is not present in mammalian samples.
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Immunogen
Synthetic peptide:
VNGHEFEIEGEGEGR
, corresponding to amino acids 22-36 of RFP from Discosoma sea anemone.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab28664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | 1/1000. Predicted molecular weight: 26 kDa. Use at a concentration of 2 µg/ml. Block membrane in 5% milk and incubate primary and secondary antibodies in milk. |
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| ICC/IF | 1/50 - 1/200. |
Target
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RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
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Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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Western blot - Anti-RFP antibody (ab28664)Western blot of recombinant RFP using ab28664.
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Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (ab28664)Immunofluorescent analysis of recombinant Red Fluorescent Protein (RFP)-transfected HeLa cells. Cells were transfected with a pCMV RFP C-Myc plasmid and 48 hours post-transfection cells were fixed with formalin and permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were blocked with 3% BSA for 15 minutes at room temperature, and then probed with ab28664 at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight® 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Datasheets and documents
References
This product has been referenced in:
- Lapierre LA et al. Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition. Mol Biol Cell 28:1088-1100 (2017). Read more (PubMed: 28228550) »
- Nakagawa N et al. APC sets the Wnt tone necessary for cerebral cortical progenitor development. Genes Dev 31:1679-1692 (2017). Read more (PubMed: 28916710) »
