Question (32128) | Anti-RFP antibody (ab28664)

Go to datasheet (ab28664)

Question

Does this antibody ab62341 recognise mcherry as well ? My colleague had asked about mcherry specific antibodies earlier, and your help team recommended ab28664 antibody - I was wondering if ab62341 would potentially be better as we want to use it in IF ?

Answer

Thank you very much for your interest in Abcam anti-RFP antibodies. ab28664 was recommended to your colleague because the homology between the immunogen and the mCherry is 100%. ab62341 was raised using the whole RFP, which has 82% homology with mCherry (see attached file). There is therefore a higher possibility that it would not recognise mCherry. To our knowledge, ab28664 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab28664 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab28664 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab28664 so please wait for my reply before ordering. 2. Purchase ab28664 either by phone, fax, or online (www.abcam.com). 3. Test it in ICC/IF. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab28664 turns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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