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Hi, I am writing in to ask about the suitability of RFP antibodies on your catalog to probe for a mCherry-tagged protein on WB. Will there be any binding issues? And for EGFP-tagged proteins, are all GFP antibodies able to detect the EGFP on WB? Many thanks.
Asked on Jan 10 2012
Thank you for your enquiry. Indeed, we do have a few RFP antibodies available that also recognise mCherry and are suitable for Western blot, as stated on each datasheet: ab28664: Click here (or use the following: https://www.abcam.com/index.html?datasheet=28664). ab105750: Click here (or use the following: https://www.abcam.com/index.html?datasheet=105750). ab109809: Click here (or use the following: https://www.abcam.com/index.html?datasheet=109809). To our knowledge, ab28664 has not been tested in specifically tested for cross-reactivity with mCherry yet. However, the immunogen shares a 100% sequence similarity with mCherry and so it is very likely that it will work. Furthermore, we do not currently have an image of ab105750 or ab109809 showing the detection of mCherry. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, test it in Western blot with mCherry and submit feedback to us in the form of an Abreview (including an image). It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and which antibody you would like to test. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering. 2. Purchase the antibody either by phone, fax, or online (www.abcam.com). 3. Test it in WB with mCherry. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. Regarding the detection of EGFP proteins, I am happy to let you know that most of our GFP antibodies also detect the enhanced GFP. This is also mentioned on each individual datasheet, such as for ab290 (Click here (or use the following: https://www.abcam.com/index.html?datasheet=290).). All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Please be reassured that all our products are covered by our Abpromise: We will happily offer Scientific Support in the event that a product is not functioning in the applications and species cited on the product datasheet (and the problem has been reported within 6 months of purchase). If it appears that the antibody is faulty, a replacement, credit or refund will be offered (https://www.abcam.com/Abpromise). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jan 10 2012