Western blot abreview for Anti-RFP antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (mApple-CD81 transfected in CHO cells)
Gel Running Conditions
Reduced Denaturing (5-20% gradient gel)
Loading amount
50 µg
Specification
mApple-CD81 transfected in CHO cells
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% non-fat dry milk in TBS-T
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Horseradish Peroxidase conjugates anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL Luminata Crescendo
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 42 kDa

Additional data

Additional Notes
Nitrocellulose membranes Lysis Buffer: 1% NP-40

Abcam user community

Verified customer

Submitted Nov 09 2015

Sign up