Immunocytochemistry/ Immunofluorescence abreview for Anti-RFP antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.3% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 10% goat serum + 3% Triton X-100
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG, Alexa Fluor 488 conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

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Submitted Dec 22 2015

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