Product nameAnti-RFP antibody
See all RFP primary antibodies
DescriptionRabbit polyclonal to RFP
Specificityab62341 recognizes RFP and has been shown to react with tdTomato.
Tested applicationsSuitable for: IHC-P, IHC-FrFl, IHC-Fr, WB, IP, ICC/IFmore details
Recombinant full length protein corresponding to RFP.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 23300543|
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 27581371|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20889618|
|WB||Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
|IP||Use a concentration of 10 - 20 µg/ml. PubMed: 19704022|
|ICC/IF||1/100. PubMed: 19474334|
RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
ab62341, at 1/100, staining chicken midbrain cells by Immunocytochemistry/ Immunofluorescence.The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody for 3 hours. An Alexa fluor® 568 goat polyclonal to rabbit Ig, diluted 1/300, was used as the secondary antibody.
All lanes : Anti-RFP antibody (ab62341) at 1/2000 dilution
All lanes : Whole cell lysate prepared from pDsRed-Monomer transfected HEK293 cells
Lysates/proteins at 20 µg per lane.
All lanes : HRP conjugated anti-rabbit at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Immunohistochemical analysis of paraffin-embedded HT29 cells tissue labeling RFP with ab62341 at 1400 dilution,followed by Goat Anti-Rabbit (HRP) (ab62341)
ab62341 staining RFP in rat oligodendrocytes transfected with recombinant human adenovirus type 5 expressing Red Fluorescent Protein (RFP) by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with formaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 5% serum for 10 minutes at 25°C. Samples were then incubated with ab62341 at a 1/300 dilution for 1 hour at 25°C. The secondary used was an Alexa Fluor® 594 goat anti-rabbit IgG (H+L), used at a 1/500 dilution.
This product has been referenced in:
- Chang CN et al. Requirement of Pitx2 for skeletal muscle homeostasis. Dev Biol 445:90-102 (2019). Read more (PubMed: 30414844) »
- Ceanga M et al. Stroke Accelerates and Uncouples Intrinsic and Synaptic Excitability Maturation of Mouse Hippocampal DCX+ Adult-Born Granule Cells. J Neurosci 39:1755-1766 (2019). Read more (PubMed: 30617211) »