Anti-RFP antibody (ab62341)
Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RFP antibody
See all RFP primary antibodies -
Description
Rabbit polyclonal to RFP -
Host species
Rabbit -
Specificity
ab62341 recognizes RFP and has been shown to react with tdTomato. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to RFP.
Database link: Q9U6Y8 -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3993 RFP (dsRed) Antibody. 3993-100 is the same size as the 100 µg size of ab62341.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (8) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 27.6 kDa.
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Notes |
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 27.6 kDa. |
Target
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Relevance
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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All lanes : Anti-RFP antibody (ab62341) at 1 µg/ml
Lane 1 : Recombinant RFP 2ng
Lane 2 : Recombinant RFP 10ng
Lane 3 : Recombinant RFP 50ng
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 27.6 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking Buffer - 3% Milk
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (290)
ab62341 has been referenced in 290 publications.
- Liau ES et al. Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons. Nat Commun 14:46 (2023). PubMed: 36596814
- von Elsner L et al. Biallelic FRA10AC1 variants cause a neurodevelopmental disorder with growth retardation. Brain 145:1551-1563 (2022). PubMed: 34694367
- Bertero A et al. A Novel Layer 4 Corticofugal Cell Type/Projection Involved in Thalamo-Cortico-Striatal Sensory Processing. J Neurosci 42:1383-1405 (2022). PubMed: 34983816
- Göz Aytürk D et al. Mouse Lines with Cre-Mediated Recombination in Retinal Amacrine Cells. eNeuro 9:N/A (2022). PubMed: 35045975
- Lama C et al. The nuclear receptor Hr46/Hr3 is required in the blood brain barrier of mature males for courtship. PLoS Genet 18:e1009519 (2022). PubMed: 35077443