Overview

  • Product name
  • Description
    Rabbit polyclonal to RFP
  • Host species
    Rabbit
  • Specificity
    ab62341 recognizes RFP and has been shown to react with tdTomato.
  • Tested applications
    Suitable for: IHC-P, IHC-FrFl, IHC-Fr, WB, IP, ICC/IFmore details
  • Immunogen

    Recombinant full length protein corresponding to RFP.

  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 23300543
IHC-FrFl Use at an assay dependent concentration. PubMed: 27581371
IHC-Fr Use at an assay dependent concentration. PubMed: 20889618
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP Use a concentration of 10 - 20 µg/ml. PubMed: 19704022
ICC/IF 1/100. PubMed: 19474334

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • ab62341, at 1/100, staining chicken midbrain cells by Immunocytochemistry/ Immunofluorescence.The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody for 3 hours. An Alexa fluor® 568 goat polyclonal to rabbit Ig, diluted 1/300, was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-RFP antibody (ab62341) at 1/2000 dilution

    All lanes : Whole cell lysate prepared from pDsRed-Monomer transfected HEK293 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated anti-rabbit at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Immunohistochemical analysis of paraffin-embedded HT29 cells tissue labeling RFP with ab62341 at 1400 dilution,followed by Goat Anti-Rabbit (HRP) (ab62341)

  • ab62341 staining RFP in rat oligodendrocytes transfected with recombinant human adenovirus type 5 expressing Red Fluorescent Protein (RFP) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with formaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 5% serum for 10 minutes at 25°C. Samples were then incubated with ab62341 at a 1/300 dilution for 1 hour at 25°C. The secondary used was an Alexa Fluor® 594 goat anti-rabbit IgG (H+L), used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Chang CN  et al. Requirement of Pitx2 for skeletal muscle homeostasis. Dev Biol 445:90-102 (2019). Read more (PubMed: 30414844) »
  • Ceanga M  et al. Stroke Accelerates and Uncouples Intrinsic and Synaptic Excitability Maturation of Mouse Hippocampal DCX+ Adult-Born Granule Cells. J Neurosci 39:1755-1766 (2019). Read more (PubMed: 30617211) »
See all 130 Publications for this product

Customer reviews and Q&As

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1-10 of 21 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Colon)
Specification
Colon
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 18 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (Mouse Epiblast stem cells expressing Dsred)
Specification
Mouse Epiblast stem cells expressing Dsred
Permeabilization
Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
Fixative
Paraformaldehyde

Miss. Yali Huang

Verified customer

Submitted Nov 29 2013

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Sample
Rat Tissue sections (pituitary gland of mRFP1 gene transgenic rat)
Specification
pituitary gland of mRFP1 gene transgenic rat
Permeabilization
Yes - Triton-X
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 30 2013

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Monkey Cell lysate - other (cos7 cell)
Specification
cos7 cell
Treatment
overexpress RFP construct
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cell)
Specification
Hela cell
Permeabilization
No
Fixative
Formaldehyde

Ms. Ya Zhou

Verified customer

Submitted Sep 19 2013

Abreviews
Application
Western blot
Sample
Human Purified protein (HEK-293T)
Gel Running Conditions
Reduced Denaturing (10% Acrylamide gel)
Loading amount
40 µg
Treatment
transfected with GFP, mCherry, and mApple N1
Specification
HEK-293T
Blocking step
OneBlock™ Western-CL Blocking Buffer as blocking agent for 40 minute(s) · Concentration: 100% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Aug 03 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Resin sections)
Sample
Mouse Tissue sections (brain tissue)
Specification
brain tissue

Abcam user community

Verified customer

Submitted Jun 15 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (HT29 cells expressing DsRed protein)
Antigen retrieval step
Enzymatic
Permeabilization
Yes - Tween-20
Specification
HT29 cells expressing DsRed protein
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 28 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
56000 cells
Treatment
Transfeceted for 24 hours
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Sep 23 2015

1-10 of 21 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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