Key features and details
- Mouse monoclonal [BV3-2] to RFP
- Suitable for: WB
- Isotype: IgG2b
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.03% Proclin 300
Constituents: 30% Glycerol (glycerin, glycerine), 0.5% BSA, PBS, 0.15% EDTA
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The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 0.5 - 4.0 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Anti-RFP antibody [BV3-2] (ab50896) at 0.5 µg/ml + 293 cells transfected with a RFP-fusion construct.
Observed band size: 41 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab50896 has been referenced in 1 publication.
- Vicencio JM et al. The inositol 1,4,5-trisphosphate receptor regulates autophagy through its interaction with Beclin 1. Cell Death Differ 16:1006-17 (2009). IP . PubMed: 19325567