Anti-RFP antibody [BV3-2] (ab50896)
![Western blot - Anti-RFP antibody [BV-F9] (ab50896)](https://www.abcam.com/ps/products/50/ab50896/Images/50896wb.jpg "Western blot - Anti-RFP antibody [BV-F9] (ab50896)")
Key features and details
- Mouse monoclonal [BV3-2] to RFP
- Suitable for: WB
- Isotype: IgG2b
Overview
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Product name
Anti-RFP antibody [BV3-2]
See all RFP primary antibodies -
Description
Mouse monoclonal [BV3-2] to RFP -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Immunogen
Recombinant Red Fluorescence Protein expressed in E. coli
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3994 Red Fluorescent Protein (dsRed) Antibody. 3994-100 is the same size as the 100 µg size of ab50896.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.03% Proclin 300
Constituents: 30% Glycerol (glycerin, glycerine), 0.5% BSA, PBS, 0.15% EDTA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
BV3-2 -
Isotype
IgG2b -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
| Notes |
|---|
Application notes
ICC/IF: Use at an assay dependent dilution.
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 0.5 - 4.0 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 0.5 - 4.0 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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Western blot - Anti-RFP antibody [BV-F9] (ab50896)Anti-RFP antibody [BV3-2] (ab50896) at 0.5 µg/ml + 293 cells transfected with a RFP-fusion construct.
Observed band size: 41 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab50896 has been referenced in 1 publication.
- Vicencio JM et al. The inositol 1,4,5-trisphosphate receptor regulates autophagy through its interaction with Beclin 1. Cell Death Differ 16:1006-17 (2009). IP . PubMed: 19325567