Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-RFP antibody [EPR18992] (ab185921)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)
  • Western blot - Anti-RFP antibody [EPR18992] (ab185921)
  • Anti-RFP antibody [EPR18992] (ab185921)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18992] to RFP
  • Suitable for: ICC/IF, WB
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-RFP antibody [EPR18992]
    See all RFP primary antibodies

  • Description

    Rabbit monoclonal [EPR18992] to RFP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Recombinant full length protein within RFP aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (Entacmaea quadricolor)
    Database link: Q8ISF8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: RFP transfected HEK-293 whole cell lysate. ICC/IF: RFP transfected HEK-293 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18992

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
WB
1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Notes
ICC/IF
1/100.
WB
1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Relevance

    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

  • Alternative names

    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)
    Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Empty vector transfected or RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling RFP with ab185921 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing positive staining after transfection of RFP on HEK-293 cells. 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR18992] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab185921 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Western blot - Anti-RFP antibody [EPR18992] (ab185921)
    Western blot - Anti-RFP antibody [EPR18992] (ab185921)
    All lanes : Anti-RFP antibody [EPR18992] (ab185921) at 1/10000 dilution

    Lane 1 : RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : Empty vector (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : Human kidney lysate
    Lane 6 : Human liver lysate
    Lane 7 : Mouse brain lysate
    Lane 8 : Mouse heart lysate
    Lane 9 : Rat brain lysate
    Lane 10 : Rat heart lysate
    Lane 11 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 12 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 13 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 14 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-RFP antibody [EPR18992] (ab185921)
    Anti-RFP antibody [EPR18992] (ab185921)

References (1)

Publishing research using ab185921? Please let us know so that we can cite the reference in this datasheet.

ab185921 has been referenced in 1 publication.

  • Xia N  et al. African Swine Fever Virus Structural Protein p17 Inhibits Cell Proliferation through ER Stress-ROS Mediated Cell Cycle Arrest. Viruses 13:N/A (2020). PubMed: 33374251

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab185921.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com