Recombinant
RabMAb

Recombinant Anti-RFP antibody [EPR18992] (ab185921)

Rabbit recombinant monoclonal RFP antibody [EPR18992]. Validated in WB, ICC/IF. Immunogen corresponding to recombinant full length protein.

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Overview

  • Product name

    Anti-RFP antibody [EPR18992]
    See all RFP primary antibodies

  • Description

    Rabbit monoclonal [EPR18992] to RFP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Immunogen

    Recombinant full length protein within RFP aa 1 to the C-terminus. The exact sequence is proprietary. (Entacmaea quadricolor)
    Database link: Q8ISF8

  • Positive control

    • WB: RFP transfected HEK-293 whole cell lysate. ICC/IF: RFP transfected HEK-293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18992

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Relevance

    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

  • Alternative names

    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)
    Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Empty vector transfected or RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling RFP with ab185921 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing positive staining after transfection of RFP on HEK-293 cells. 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR18992] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab185921 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Western blot - Anti-RFP antibody [EPR18992] (ab185921)
    Western blot - Anti-RFP antibody [EPR18992] (ab185921)
    All lanes : Anti-RFP antibody [EPR18992] (ab185921) at 1/10000 dilution

    Lane 1 : RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : Empty vector (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : Human kidney lysate
    Lane 6 : Human liver lysate
    Lane 7 : Mouse brain lysate
    Lane 8 : Mouse heart lysate
    Lane 9 : Rat brain lysate
    Lane 10 : Rat heart lysate
    Lane 11 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 12 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 13 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 14 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab185921 has not yet been referenced specifically in any publications.

Publishing research using ab185921? Please let us know so that we can cite the reference in this datasheet.

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