Anti-RFP antibody [EPR18992] (ab185921)
Rabbit recombinant monoclonal RFP antibody [EPR18992]. Validated in WB, ICC/IF. Immunogen corresponding to recombinant full length protein.
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-RFP antibody [EPR18992]
See all RFP primary antibodies -
DescriptionRabbit monoclonal [EPR18992] to RFP
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Immunogen
Recombinant full length protein within RFP aa 1 to the C-terminus. The exact sequence is proprietary. (Entacmaea quadricolor)
Database link: Q8ISF8 -
Positive control
- WB: RFP transfected HEK-293 whole cell lysate. ICC/IF: RFP transfected HEK-293 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR18992
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab185921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
Target
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RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
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Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)](https://www.abcam.com/ps/products/185/ab185921/Images/ab185921-253140-185921IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Empty vector transfected or RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling RFP with ab185921 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing positive staining after transfection of RFP on HEK-293 cells.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR18992] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab185921 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
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![Western blot - Anti-RFP antibody [EPR18992] (ab185921)](https://www.abcam.com/ps/products/185/ab185921/Images/ab185921-253141-185921WB.jpg)
Western blot - Anti-RFP antibody [EPR18992] (ab185921)All lanes : Anti-RFP antibody [EPR18992] (ab185921) at 1/10000 dilution
Lane 1 : RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Empty vector (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : Human kidney lysate
Lane 6 : Human liver lysate
Lane 7 : Mouse brain lysate
Lane 8 : Mouse heart lysate
Lane 9 : Rat brain lysate
Lane 10 : Rat heart lysate
Lane 11 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 12 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 13 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 14 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
References
ab185921 has not yet been referenced specifically in any publications.
