Overview

  • Product name
    Anti-RFP antibody (FITC)
    See all RFP primary antibodies
  • Description
    Rabbit polyclonal to RFP (FITC)
  • Host species
    Rabbit
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab34764 detects RFP tagged proteins. No reaction was observed against serum proteins.Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry”.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, ELISAmore details
  • Immunogen

    Fusion protein corresponding to RFP. derived from the mushroom polyp coral Discosoma

  • General notes

    3.4 moles FITC per mole of rabbit IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    affinity purification was followed by solid phase adsorption to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500 - 1/2500.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/2500.
WB 1/10000. Detects a band of approximately 27 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • All lanes : Anti-RFP antibody (FITC) (ab34764) at 1/2500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

References

This product has been referenced in:
  • Donnelly JM  et al. Sonic hedgehog mediates the proliferation and recruitment of transformed mesenchymal stem cells to the stomach. PLoS One 8:e75225 (2013). Read more (PubMed: 24069395) »
  • Starremans PG  et al. A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1. Kidney Int 73:1394-405 (2008). Read more (PubMed: 18385665) »
See all 3 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

We have not yet tested the FITC conjugated anti RFP antibody against GFP, but this antibody did not show significant amounts of specific binding to BFP.

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Answer

DISCOUNT CODE: ABTRIAL-xxxxx
Expiration date: 04/04/12
Value: €410.00

I am very pleased to hear you would like to accept our offer and test ab34771 in Flow Cytometry. This code will give you €410.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flow cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


It is correct, that you do not have to mention the code with your order of ab34771. The code will be activated by submitting the Abreview and needs to be mentioned with the next order.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you for your answer.

I would be pleased to offer you the AbTrial for the ab34771 to use this antibody in FlowCytometry. If you decide to order this antibody please let me now in advance so that I can provide you with the code.

You will receive the whole value of the antibody you are testing to order a new product. That means, if you decide to test the ab34771 you will receive a code with a value of €410.00. The code will be activated after you submit the Abreview.

Please let me know about your decision. I look forward hearing from you.

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Answer

Thank you for contacting us.

In this case I would recommend to use the Anti-RFP antibody (ab62341). If it is suitable for your experiment you could use a secondary antibody to detect the Anti RFP antibody.

If the conjugate needs to be on the primary antibody I would recommend to have a look at our conjugation kits https://www.abcam.com/index.html?pageconfig=resource&rid=13148.

Because the Anti-RFP antibody (ab62341) has not been tested for flow cytometry I would am pleased to offer you the AbTrial for the use of this product in flow cytometry. By participating in our AbTrial program you can use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting me know you are interested in testing this product.

2. I will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

I hope I could be of help and I look forward hearing from you about your decision regarding the AbTrial offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for your enquiry.

This Our RFP antibody isdesigned to detect all RFP variants from Discosoma. I am sorry we do not have specific data for the the use of this product with DS Red express in Flow Cytometry, however in theory it would be expected to work well.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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Question
Answer

Thank you for contacting us with your question.

We have not specifically tested the antibody against mRFP1, but it is designed to react with RFP variants such as mRFP1. If you do have any problems using this antibody with mRPF1, please let me know as it will be covered under our Abpromise guarantee.

I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (whole embryo)
Specification
whole embryo
Fixative
Paraformaldehyde
Permeabilization
Yes - PBT
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Ms. Vanja Stankic

Verified customer

Submitted Jan 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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