Overview

  • Product name
    Anti-RFP antibody (FITC)
    See all RFP primary antibodies
  • Description
    Rabbit polyclonal to RFP (FITC)
  • Host species
    Rabbit
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab34764 detects RFP tagged proteins. No reaction was observed against serum proteins.Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry”.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, ELISAmore details
  • Immunogen

    Fusion protein corresponding to RFP. derived from the mushroom polyp coral Discosoma

  • General notes

    3.4 moles FITC per mole of rabbit IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    affinity purification was followed by solid phase adsorption to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500 - 1/2500.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/2500.
WB 1/10000. Detects a band of approximately 27 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • All lanes : Anti-RFP antibody (FITC) (ab34764) at 1/2500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

References

This product has been referenced in:
  • Saxena V  et al. Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function. Sci Rep 9:545 (2019). Read more (PubMed: 30679625) »
  • Donnelly JM  et al. Sonic hedgehog mediates the proliferation and recruitment of transformed mesenchymal stem cells to the stomach. PLoS One 8:e75225 (2013). Read more (PubMed: 24069395) »
See all 4 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (whole embryo)
Specification
whole embryo
Fixative
Paraformaldehyde
Permeabilization
Yes - PBT
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Ms. Vanja Stankic

Verified customer

Submitted Jan 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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