Overview

  • Product name
    Anti-RFP antibody (HRP)
    See all RFP primary antibodies
  • Description
    Rabbit polyclonal to RFP (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Specificity
    ab34767 is designed to detect RFP and its variants.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Fusion protein The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    immunoaffinity chromatography was followed by solid phase adsorption to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000. Detects a band of approximately 27 kDa.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • All lanes : Anti-RFP antibody (HRP) (ab34767) at 1/2500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

References

This product has been referenced in:
  • Thevenard J  et al. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS One 9:e103839 (2014). WB . Read more (PubMed: 25075518) »
  • Chen XW  et al. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Elife 2:e00444 (2013). Read more (PubMed: 23580231) »
See all 4 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Western blot
Sample
Nicotiana benthamiana Tissue lysate - whole (Total protein extract from infiltrated leaves as i)
Gel Running Conditions
Reduced Denaturing (15% acrylamide)
Loading amount
20 µg
Specification
Total protein extract from infiltrated leaves as i
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Emily Breeze

Verified customer

Submitted Sep 28 2017

Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (E. coli expressing mCherry)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
30 cells
Specification
E. coli expressing mCherry
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 20 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (Prostate)
Specification
Prostate
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 24 2013

Answer

Thank you very much for your interest in ab34767.

To our knowledge, ab34767 has not been tested in tdTomato, however based on sequence homology there is a good chance that it will work. Therefore, I can offer a discount off a future purchase if you buy ab34767 now, test it in tdTomato and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab34767 in tdTomato. I will then send a discount code. This code must be issued before purchasing ab34767 so please wait for my reply before ordering.

2. Purchase ab34767 either by phone, fax, or online (www.abcam.com).

3. Test it in tdTomato.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab34767 turns out to be unsuitable for tdTomato you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your reply. I have sent a free vial of ab34767 to replace ab28664. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I have also issued a credit note for ab6722. The credit note may be used in one of the following ways: (1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding. Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge. To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Answer

Thank you for your email. I would be happy to send a free vial of ab34767 (to replace ab28664) aswell as credit you for the secondary antibody purchased. Do you have the secondary product code and date of order? I need to find the original order to process the credit. Looking forward to your reply.

Read More

Answer

Thank you for the further information. My colleague Kate is currently out of the office, so I will be managing this case. I am sorry to hear you have been experincing problems with ab28664. I would be happy to send you a replacment of another product, ab34767 may be of interest as it is conjugated with HRP: Click here (or use the following: https://www.abcam.com/index.html?datasheet=34767). Please let me know whether you would like to receive this antibody as a replacement. Reviewing the protocol information, I would be careful about storing the supernatant from the protein extraction at 4C, -20 may be the most appropriate storage temperature to prevent protein degradation. It would also be interesting if there is another method to confirm that the proteins are expressed. Do you have a control protein that can be expressed at the same time and testing with another antibody? This would act as a positive control. I look forward to your reply.

Read More

Answer

Thank you for your enquiry. Yes, ab28664 has been tested for Western blotting and will demonstrate the expression of your DSred fusion protein. But I recommend ab34767 which is an HRP conjugate, removing the need for a secondary detection antibody. It can be used more dilute than ab28664, making it cheaper per blot. It has been tested for ELISA, which will be a more sensitive assay for the purposes of normalization than Western blot densitometry, though Western blot will demonstrate the specificity of the antibody, revealed by a single band for the fusion protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your recent technical enquiry. Unfortunately, we do not have information regarding the reactivity of this product with mcherry. It has only been shown to detect RFP from Discosoma. I am sorry I can't be of more assistance in this occasion but should you require any further information, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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