Overview

  • Product name
    Anti-RFP antibody (HRP)
    See all RFP primary antibodies
  • Description
    Rabbit polyclonal to RFP (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Specificity
    ab34767 is designed to detect RFP and its variants.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Fusion protein The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    immunoaffinity chromatography was followed by solid phase adsorption to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000. Detects a band of approximately 27 kDa.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • All lanes : Anti-RFP antibody (HRP) (ab34767) at 1/2500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

References

This product has been referenced in:
  • Thevenard J  et al. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS One 9:e103839 (2014). WB . Read more (PubMed: 25075518) »
  • Chen XW  et al. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Elife 2:e00444 (2013). Read more (PubMed: 23580231) »
See all 4 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-3 of 3 Abreviews

Application
Western blot
Sample
Nicotiana benthamiana Tissue lysate - whole (Total protein extract from infiltrated leaves as i)
Gel Running Conditions
Reduced Denaturing (15% acrylamide)
Loading amount
20 µg
Specification
Total protein extract from infiltrated leaves as i
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Emily Breeze

Verified customer

Submitted Sep 28 2017

Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (E. coli expressing mCherry)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
30 cells
Specification
E. coli expressing mCherry
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 20 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (Prostate)
Specification
Prostate
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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