Immunocytochemistry/ Immunofluorescence abreview for Anti-RFP antibody (HRP)

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (Prostate)
Specification
Prostate
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG AlexaFluor 488 - Invitrogen
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
We used the Anti-RFP antibody to stain the dorsolateral lobe of a C57 and Tdtomato mouse prostate to determine if the Anti-RFP antibody would stain the Tdtomato mouse, as there is not currently an anti-Tdtomato antibody available. We found slightly higher staining in the Tdtomato mouse than in the C57 mouse. We also found that a 1:100 concentration yielded a high background in the C57 mouse, but a lower concentration showed no staining in the Tdtomato mouse. Green = Anti-RFP, red = probasin, blue = Hoechst.

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Submitted Jul 24 2013

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