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Escherichia coli Cell lysate - whole cell (E. coli expressing mCherry)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
E. coli expressing mCherry
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 5% nonfat milk
E. coli (DH5 alpha) without the plasmid gave no bands.
12 minute(s) and 0 second(s)
E. coli (DH5 alpha) expressing mCherry gave one crisp band at 27kDa.
If you are looking for an antibody against mCherry that is directly conjugated to HRP, this works well on Western blots.
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Submitted Aug 20 2015