Western blot abreview for Anti-RFP antibody (HRP)

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Abreviews
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (E. coli expressing mCherry)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
30 cells
Specification
E. coli expressing mCherry
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 5% nonfat milk
Dilution
1/2500

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Negative control
E. coli (DH5 alpha) without the plasmid gave no bands.
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 27 kDa
Positive control
E. coli (DH5 alpha) expressing mCherry gave one crisp band at 27kDa.

Additional data

Additional Notes
If you are looking for an antibody against mCherry that is directly conjugated to HRP, this works well on Western blots.

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Submitted Aug 20 2015

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