Western blot abreview for Anti-RFP antibody (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Nicotiana benthamiana Tissue lysate - whole (Total protein extract from infiltrated leaves as i)
Gel Running Conditions
Reduced Denaturing (15% acrylamide)
Loading amount
20 µg
Specification
Total protein extract from infiltrated leaves as i
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk in TBS Tween
Dilution
1/10000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Negative control
A GFP-tagged protein (73 kDa with GFP)
Exposure
40 minute(s) and 0 second(s)
Bands
Specific: 73 or 57 or 116 kDa Non-specific: N/A kDa
Positive control
3 different RFP-tagged proteins (57, 73 or 116 kDa with RFP)

Additional data

Additional Notes
This blot was previously labelled with anti-GFP antibody, stripped with SDS and re-probed with the anti-RFP antibody. 1. Negative control (L1-GFP, 73kDa) 2. Ladder (11, 17, 20, 25 [major band], 35, 48, 63, 75 [major band], 100, 135 kDa) 3. L1-RFP (73 kDa) 4. RFP-R2 (57 kDa) 5. L1-RFP (73 kDa) 6. RFP-R3 (116 kDa) 7. L1-RFP (73 kDa) 8. RFP-R2 (57 kDa) 9. RFP-R2 (57 kDa)

Emily Breeze

Verified customer

Submitted Sep 28 2017

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