• Product name
    Anti-RFP antibody [RF5R]
    See all RFP primary antibodies
  • Description
    Mouse monoclonal [RF5R] to RFP
  • Host species
  • Specificity
    Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DsRed, mCherry, mOrange, etc.
  • Tested applications
    Suitable for: WB, ELISA, Dot blot, IPmore details
  • Species reactivity
    Reacts with: Sea anenome
  • Immunogen

    Synthetic peptide corresponding to Sea anenome RFP (N terminal) conjugated to keyhole limpet haemocyanin.


  • Positive control
    • Transfected HEK293 cell lysate.
  • General notes

    Store at 4°C (add 0.1% NaN3) for several days to weeks.

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab125244 is purified by protein A affinity chromatography from TCS
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab125244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Database links
    • Alternative names
      • DsRed antibody
      • GFP like chromoprotein antibody
      • red fluorescent protein antibody
      • RFP antibody


    • All lanes : Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution

      Lane 1 : HEK293 cell lysate, non-transfected
      Lane 2 : HEK293 cell lysate, Turbo-RFP-transfected
      Lane 3 : HEK293 cell lysate, DsRed-transfected

      Developed using the ECL technique.

      Predicted band size: 26 kDa


    This product has been referenced in:
    • Shimo T  et al. Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening. PLoS One 13:e0197373 (2018). Read more (PubMed: 29768479) »
    • Piacentino ML & Bronner ME Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction. PLoS Biol 16:e2004425 (2018). Read more (PubMed: 29949573) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    IHC - Wholemount
    Tribolium castaneum Embryo (Wholemount)

    Abcam user community

    Verified customer

    Submitted Aug 16 2013


    Vielen Dank für Ihren Anruf.

    Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

    Ich habe das Datenblatt noch einmal ueberprueft, und ich denke, wir haben uns missverstanden! Gehe ich richtig in der Annahme, dass Sie ein Protein mit dem RFP getagget haben, und dies hat nicht funktioniert.

    Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll. Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und möglichst Veränderungsvorschläge machen, die Ihre Ergebnisse verbessern werden. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und er innerhalb der letzten 180 Tage gekauft wurde, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken.

    Ich freue mich, bald wieder von Ihnen zu hören.

    Read More


    Thank you for contacting us.
    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
    I look forward to receiving your reply.

    Read More


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