Anti-RFP antibody [RF5R] (ab125244)
![Western blot - Anti-RFP antibody [RF5R] (ab125244)](https://www.abcam.com/ps/products/125/ab125244/Images/ab125244-139414-anti-rfp-antibody-rf5r-western-blot.jpg "Western blot - Anti-RFP antibody [RF5R] (ab125244)")
Key features and details
- Mouse monoclonal [RF5R] to RFP
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-RFP antibody [RF5R]
See all RFP primary antibodies -
Description
Mouse monoclonal [RF5R] to RFP -
Host species
Mouse -
Specificity
Recognizes native and denatured forms of RFP and its turbo-RFP, DsRed variants
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Transfected HEK293 cell lysate.
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General notes
Store at 4°C (add 0.1% NaN3) for several days to weeks.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab125244 is purified by protein A affinity chromatography from TCS -
Clonality
Monoclonal -
Clone number
RF5R -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins.
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| Notes |
|---|
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WB
1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins. |
Target
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Relevance
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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Western blot - Anti-RFP antibody [RF5R] (ab125244)All lanes : Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution
Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, Turbo-RFP-transfected
Lane 3 : HEK293 cell lysate, DsRed-transfected
Developed using the ECL technique.
Predicted band size: 26 kDa
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab125244 has been referenced in 9 publications.
- Su M et al. Loss of Calretinin in L5a impairs the formation of the barrel cortex leading to abnormal whisker-mediated behaviors. Mol Brain 14:67 (2021). PubMed: 33845857
- Kueck T et al. Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. J Virol 93:N/A (2019). PubMed: 31578292
- Seishima R et al. Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development. Nat Commun 10:5378 (2019). PubMed: 31772170
- Dimri S et al. Dynamic monitoring of STAT3 activation in live cells using a novel STAT3 Phospho-BRET sensor. Am J Nucl Med Mol Imaging 9:321-334 (2019). PubMed: 31976162
- Shimo T et al. Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening. PLoS One 13:e0197373 (2018). PubMed: 29768479
- Piacentino ML & Bronner ME Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction. PLoS Biol 16:e2004425 (2018). PubMed: 29949573
- Golfetto O et al. A Platform To Enhance Quantitative Single Molecule Localization Microscopy. J Am Chem Soc 140:12785-12797 (2018). PubMed: 30256630
- Klisch TJ et al. Jak2-mediated phosphorylation of Atoh1 is critical for medulloblastoma growth. Elife 6:N/A (2017). PubMed: 29168692
- Kreko-Pierce T & Eaton BA The Drosophila LC8 homolog cut up specifies the axonal transport of proteasomes. J Cell Sci 130:3388-3398 (2017). PubMed: 28808087
