Overview

  • Product name
    Anti-RFP antibody [RFP.H8]
    See all RFP primary antibodies
  • Description
    Mouse monoclonal [RFP.H8] to RFP
  • Host species
    Mouse
  • Specificity
    ab109809 recognizes native and denatured forms of red fluorescent protein (RFP). It will also recognize tag-RFP, turbo-RFP, DeRed, mCherry, and mOrange.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to RFP conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH from the N-terminal of RFP protein from Discosoma anemone.

  • Positive control
    • WB: HEK-293T cell lysate transfected with DsRed Expression.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    RFP.H8
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 26 kDa.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • All lanes : Anti-RFP antibody [RFP.H8] (ab109809) at 1/1000 dilution

    Lane 1 : Molecular ladder
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate with DsRed Expression at 1 µg
    Lane 3 : HEK-293 cell lysate with DsRed Expression at 5 µg
    Lane 4 : HEK-293 cell lysate with DsRed Expression at 10 µg

    Predicted band size: 26 kDa

  • All lanes : Anti-RFP antibody [RFP.H8] (ab109809) at 1/1000 dilution

    Lane 1 : HEK293 cell untransfected control
    Lane 2 : HEK293 cells transfected with Turbo-RFP
    Lane 3 : HEK293 transfected with DeRed

    Predicted band size: 26 kDa

References

ab109809 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Mouse Embryo (Brain)
Specification
Brain

Mr. Amey Mukim

Verified customer

Submitted Mar 06 2012

Answer

Thank you for contacting Abcam.

We have very few antibodies against RFP that are not raised in rabbit. Of these, the best candidate for having no reaction with GFP isab109809 as it is raised against the N-terminal region of the protein which has very poor homology with GFP. However, none of these products have been expressly tested agianst GFP.

I hope that you find this information helpful. Please contact me should you have any questions.

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Question
Answer

DISCOUNT CODE: **** Expiration date: May 12, 2012 I am very pleased to hear you would like to accept our offer and test ab109809 in ICC (immunocytochemistry). This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. (If you are not testing the antibody in ICC, on cell cultures, but in IHC on tissue sections instead, please let me know). For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardles of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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