Overview

  • Product name
    Anti-RFP antibody [RFP.H8]
    See all RFP primary antibodies
  • Description
    Mouse monoclonal [RFP.H8] to RFP
  • Host species
    Mouse
  • Specificity
    ab109809 recognizes native and denatured forms of red fluorescent protein (RFP). It will also recognize tag-RFP, turbo-RFP, DeRed, mCherry, and mOrange.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to RFP conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH from the N-terminal of RFP protein from Discosoma anemone.

  • Positive control
    • WB: HEK-293T cell lysate transfected with DsRed Expression.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    RFP.H8
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 26 kDa.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • All lanes : Anti-RFP antibody [RFP.H8] (ab109809) at 1/1000 dilution

    Lane 1 : Molecular ladder
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate with DsRed Expression at 1 µg
    Lane 3 : HEK-293 cell lysate with DsRed Expression at 5 µg
    Lane 4 : HEK-293 cell lysate with DsRed Expression at 10 µg

    Predicted band size: 26 kDa

  • All lanes : Anti-RFP antibody [RFP.H8] (ab109809) at 1/1000 dilution

    Lane 1 : HEK293 cell untransfected control
    Lane 2 : HEK293 cells transfected with Turbo-RFP
    Lane 3 : HEK293 transfected with DeRed

    Predicted band size: 26 kDa

References

ab109809 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
IHC - Wholemount
Sample
Mouse Embryo (Brain)
Specification
Brain

Mr. Amey Mukim

Verified customer

Submitted Mar 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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