Key features and details
- Rabbit polyclonal to RFPL2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RFPL2 antibody
See all RFPL2 primary antibodies
DescriptionRabbit polyclonal to RFPL2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human RFPL2 aa 99-378.
SSCPVCSDYLEKPMSLECGCAVCLKCINSLQKEPHGEDLLCCCSSMVSRK NKIRRNRQLERLASHIKELEPKLKKILQMNPRMRKFQVDMTLDANTANNF LLISDDLRSVRSGRIRQNRQDLAERFDVSVCILGSPRFTCGRHCWEVDVG TSTEWDLGVCRESVHRKGRIQLTTELGFWTVSLRDGGRLSATTVPLTFLF VDRKLQRVGIFLDMGMQNVSFFDAESGSHVYTFRSVSAEEPLRPFLAPSV PPNGDQGVLSICPLMNSGTTDAPVRPGEAK
Database link: O75678
- WB: U-87 MG and MCF7 whole cell lysates. IHC-P: Human brain and prostate tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 32,42 kDa).|
|IHC-P||1/20 - 1/200.|
Tissue specificitySeems to be expressed in prostate and less abundantly in adult brain, fetal liver, and fetal kidney.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- Ret finger protein like 2 antibody
- Ret finger protein-like 2 antibody
- RFPL 2 antibody
All lanes : Anti-RFPL2 antibody (ab230087) at 1/1000 dilution
Lane 1 : U87-MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 32,42 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Paraffin-embedded human brain tissue stained for RFPL2 using ab230087 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for RFPL2 using ab230087 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230087 has not yet been referenced specifically in any publications.