Key features and details
- Guinea pig polyclonal to RFRP
- Suitable for: IHC-Fr
- Reacts with: Sheep
- Isotype: IgG
Product nameAnti-RFRP antibody
See all RFRP primary antibodies
DescriptionGuinea pig polyclonal to RFRP
Host speciesGuinea pig
Tested applicationsSuitable for: IHC-Frmore details
Species reactivityReacts with: Sheep
Predicted to work with: Human
- IHC-P: Sheep hypothalamus tissue.
Previously labelled as NPVF.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
FormLyophilized:Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. After reconstitution, aliquot and store at -20°C for a higher stability.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Concentration information loading...
Our Abpromise guarantee covers the use of ab228496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionNeuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia.
Tissue specificityIsoform 1 is specifically expressed in the retina. Neuropeptides RFRP-1 and NPVF are detected in the hypothalamus.
Sequence similaritiesBelongs to the FARP (FMRFamide related peptide) family.
- Information by UniProt
- C7orf9 antibody
- Neuropeptide NPSF antibody
- Neuropeptide NPVF antibody
4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis.
4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with Kisspeptin.
4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with Prolactin-releasing peptide (PrRP).
4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with NFF peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228496 has not yet been referenced specifically in any publications.