Anti-RFWD3 antibody (ab138030)
Key features and details
- Rabbit polyclonal to RFWD3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RFWD3 antibody
See all RFWD3 primary antibodies -
Description
Rabbit polyclonal to RFWD3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to an internal region of Human RFWD3.
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Positive control
- HepG2 whole cell lysate (ab7900).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab138030 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 85 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 85 kDa. |
Target
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Function
E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger.
Contains 3 WD repeats. -
Post-translational
modificationsPhosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55159 Human
- Omim: 614151 Human
- SwissProt: Q6PCD5 Human
- Unigene: 567525 Human
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Alternative names
- BC027246 antibody
- E3 ubiquitin protein ligase RFWD3 antibody
- E3 ubiquitin-protein ligase RFWD3 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab138030 has been referenced in 5 publications.
- Aminu B et al. Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity. Biomolecules 12:N/A (2022). PubMed: 35454069
- Gallina I et al. The ubiquitin ligase RFWD3 is required for translesion DNA synthesis. Mol Cell 81:442-458.e9 (2021). PubMed: 33321094
- Hsu RYC et al. The E3 ligase RFWD3 stabilizes ORC in a p53-dependent manner. Cell Cycle 19:2927-2938 (2020). PubMed: 33044890
- Feeney L et al. RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for Interstrand Crosslink Repair and Human Health. Mol Cell 66:610-621.e4 (2017). PubMed: 28575657
- Inano S et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell 66:622-634.e8 (2017). PubMed: 28575658