Overview

  • Product name

  • Description

    Rabbit polyclonal to RFWD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to an internal region of Human RFWD3.

  • Positive control

    • HepG2 whole cell lysate (ab7900).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138030 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab138030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 85 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
    Contains 3 WD repeats.
  • Post-translational
    modifications

    Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BC027246 antibody
    • E3 ubiquitin protein ligase RFWD3 antibody
    • E3 ubiquitin-protein ligase RFWD3 antibody
    • FLJ10520 antibody
    • MGC27888 antibody
    • RFWD3 antibody
    • RFWD3_HUMAN antibody
    • Ring finger and WD repeat domain 3 antibody
    • RING finger and WD repeat domain containing protein 3 antibody
    • RING finger and WD repeat domain-containing protein 3 antibody
    • RING finger protein 201 antibody
    • RNF201 antibody
    see all

Images

  • Anti-RFWD3 antibody (ab138030) at 1/500 dilution + HepG2 cell lysate at 30 µg

    Predicted band size: 85 kDa

References

This product has been referenced in:

  • Feeney L  et al. RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for Interstrand Crosslink Repair and Human Health. Mol Cell 66:610-621.e4 (2017). Read more (PubMed: 28575657) »
  • Inano S  et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell 66:622-634.e8 (2017). Read more (PubMed: 28575658) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (6%)
Sample
Human Cell lysate - whole cell (HeLa cell line)
Specification
HeLa cell line
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Dec 12 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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