Key features and details
- Rabbit polyclonal to RFWD3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RFWD3 antibody
See all RFWD3 primary antibodies
DescriptionRabbit polyclonal to RFWD3
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Immunogen corresponds to a region within amino acids 75-125 of Human RFWD3 (NP_060594.3).
- Hela whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionE3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-type zinc finger.
Contains 3 WD repeats.
modificationsPhosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53.
- Information by UniProt
- BC027246 antibody
- E3 ubiquitin protein ligase RFWD3 antibody
- E3 ubiquitin-protein ligase RFWD3 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab99306 has been referenced in 1 publication.
- Lai Y et al. HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation. Sci Rep 9:14257 (2019). PubMed: 31582797