The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter.
Belongs to the RFX family. Contains 1 RFX-type winged-helix DNA-binding domain.
Detection of RFX1 by Western Blot of Immunprecipitate.
anti-RFX1 at 0.4µg/ml staining RFX1 in HeLa whole cell lysate immunoprecipitated using ab110927 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.