Anti-RFX2 antibody (ab79241)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RFX2 antibody
See all RFX2 primary antibodies -
Description
Rabbit polyclonal to RFX2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human RFX2 aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab89390) -
Positive control
- This antibody gave a positive signal in the HeLa nuclear whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab79241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 80 kDa). |
Target
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Sequence similarities
Belongs to the RFX family.
Contains 1 RFX-type winged-helix DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5990 Human
- Omim: 142765 Human
- SwissProt: P48378 Human
- Unigene: 465709 Human
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Alternative names
- DNA-binding protein RFX2 antibody
- HLA class II regulatory factor RFX2 antibody
- regulatory factor X 2 (influences HLA class II expression) antibody
see all
Images
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Western blot - Anti-RFX2 antibody (ab79241)Anti-RFX2 antibody (ab79241) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
Datasheets and documents
References
ab79241 has not yet been referenced specifically in any publications.
